The invention provides a pharmaceutical composition for treating diabetes. The pharmaceutical composition comprises a medicament prepared by glimepiride and geniposide as main components, by weight ratio: 1-10 parts of glimepiride, and 1-20 parts of geniposide. The invention also provides a preparation method and applications of the pharmaceutical composition. With compatible use of glimepiride and geniposide, the pharmaceutical can play a synergistic pharmacological function, is convenient for clinical application, and provides a new choice for clinics.

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