The invention discloses compound 23 Oleanolic Acid and preparation method thereof and the application in preparing glycosidase inhibitor fall in 20 trihydroxyies-29-.The present invention extracts separated potent a-glucosidase inhibitor from Three Akebia Decne Species vegetable material source is abundant extract preparation method's easy handling and also can make plant itself be utilized for a long time without destruction when adopting fruit to extract can increase economic efficiency environmentally friendly again and this monomeric compound is stable easy to store.Pharmacological evaluation shows compound 23 and the a-Glucosidase inhibitor specific activity one line diabetes medicament acarbose that olea-12-alkene-28-acid falls in 20-trihydroxy--29-is stronger is expected to be further development of the medicine of clinical treatment type II diabetes promise well.

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