The present invention relates to the extracting method of alpha-amylase inhibitor aim to provide a kind of method of extracting alpha-amylase inhibitor from tea fruit skin.The step of the method comprises: the tea fruit skin that takes crushed after being dried adds dehydrated alcohol soak the centrifugal tea fruit skin that obtains crossing through soaked in absolute ethyl alcohol then add ethanol water the centrifugal supernatant that obtains after supersound extraction add successively again acetone chloroform to extract and obtain raffinate in raffinate add n-butanol extraction to obtain butanol extraction liquid last vacuum drying obtains alpha-amylase inhibitor.The present invention has extracted a kind of alpha-amylase inhibitor by supersound extraction and polarity screening from tea fruit skin and alpha-amylase inhibitor prepared by the present invention is a kind of natural alpha-amylase inhibitor inhibition to α-amylase is better than acarbose in addition technology of the present invention can effectively improve the economic benefit of tea fruit skin resource.

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