The invention discloses a kind of netilmicin sulfate freeze-dried powder and preparation method thereof; Netilmicin sulfate and additives are added water be made into the netilmicin sulfate suspension; Get the netilmicin sulfate freeze-dried powder through lyophilization; Wherein additives are enclose molecule and pH regulator agent and the enclose molecule is selected from Pseudobulbus Bletillae polysaccharose beta-schardinger dextrin- carbamide thiourea beta-cyclodextrin derivative deoxycholic acid and starch and the pH regulator agent is selected from sodium carbonate sodium bicarbonate ammonia sulphuric acid and phosphoric acid.Adjuvant required for the present invention is few and preparation technology is simple especially freeze-dry process is carried out screening and optimizing has characteristics such as stability is strong bioavailability is high production cost is low and quality safety is reliable.

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