The invention discloses preparation method and the application of a kind of benzyl penicilloic acid artificial antigen and antibody thereof is a kind of method that simple and fast detects penicillin G and degradation product benzyl penicilloic acid thereof effectively.The objective of the invention is by designing synthetic haptens and artificial antigen with penicillins veterinary drug penicillin G and degradation product benzyl penicilloic acid thereof of 6 one hexosamine molecular structures and beta-lactam nucleus molecular structure preparation is for the specific antibody of penicillin G and degradation product benzyl penicilloic acid thereof; Unique distinction is to have given prominence to this class veterinary drug molecular specificity antigenic determinant has overcome again the difficulty of chemosynthesis and immune animal is induced and produces the very high specific antibody of affinity.

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