The invention belongs to field of pharmaceutical preparations a kind of teniposide infatmul pre-emulsifying concentrated solution and preparation method thereof is disclosed injection fat emulsion pre-emulsifying concentrated solution of the present invention is by teniposide oil phase phospholipid the injection organic solvent is formed each weight percentages of components is as follows: teniposide 0.01-10% oil phase 0%-20% phosphatidase 11 0-80% injection organic solvent 10-99% preparation method is: the active component teniposide is dissolved in earlier in organic solvent or oil phase or their mixed liquor add other component in the prescription again can form transparent clarifying pre-emulsifying concentrated solution after stirring makes mix homogeneously.

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