biotechnology. SUBSTANCE invention relates to a novel method of preparing ajmaline in Rauwolfia serpentica tissue culturing. Rauwolfia tissue culturing is carried out in nutrient medium in the presence of bis-(hydroxymethyl)-phosphinic acid melamine salt (melaphene) taken in concentrations 1x10-3-1x10-5 g/l. The use of bis-(hydroxymethyl)-phosphinic acid melamine salt (melaphene) ensures to carry out the process both in dark and in light. Culturing Rauwolfia serpentina in nutrient medium in the ...

The use of a compound of the formula (1):or a pharmaceutically acceptable salt thereof in manufacturing a composition for reducing intraocular pressure of mammals suffering from abnormally elevated intraocular pressure is provided. A pharmaceutical formulation containing the compound of the formula (1) as an essential component is also provided.

1. Nb -quaternary compounds of the general Formula I see diagramm : EP0182271P17F1 wherein R stands for an alkyl group with 1 - 6 carbon atoms which may optionally be substituted by hydroxy or halogen on a carbon atom which is not bonded to nitrogen for an alkenyl group with 3 to 6 carbon atoms the double bond of which is not directly bonded to the nitrogen atom for a phenylalkyl group with 2 to 3 carbon atoms in the alkylene chain which may optionally be substituted in the phenyl ring by alko...