The invention relates to methods for protecting and/or treating a mammal at risk of acquiring a condition associated with bacteria that produce a calmodulin exotoxin a metalloproteinase exotoxin or both by administering a non-antibacterial tetracycline formulation.

The present invention features materials and methods for reducing the risk of cardiovascular disease in postmenopausal or perimenopausal women. More specifically these methods can be used in women who generally have no apparent cardiovascular disease. We describe herein methods of administering non-antibacterial tetracycline or a sub-antimicrobial amount of antibacterial tetracyclines or tetracycline formulations and their use in reducing the risk that cardiovascular disease will develop in a su...

The invention is a method of enhancing endogenous interleukin-10 production in mammalian cells and tissues which includes administering an effective amount of a tetracycline derivative. The method also includes enhancing interleukin-10 production by administering an effective amount of the tetracycline derivative to a mammal. Preferred tetracycline compounds are tetracycline compounds which have been modified to reduce or eliminate their antimicrobial activity. The method can be used to treat me...

The invention is a method of inhibiting the production of nitric oxide (NO) in an in vivo in vitro or ex vivo biological system. The method employs a tetracycline compound to inhibit the production of NO and/or to inhibit the expression or activity of an inducible isoform ot nitric oxide synthase (iNOS). Preferably the tetraclycine compou nd has inhibitory activity for metalloproteinases. Also it is preferred that the tetracycline compound is provided to the biological syste m in an amount which...

The invention is a method of inhibiting cancer growth by inhibiting cellula r proliferation invasiveness or metastasis or by inducing cytotoxicity against cancer in mammals. The method employs 6-demethyl-6-deox y- 4-de(dimethylamino)tetracycline (CMT-3) and other functionally related chemically modified preferably non-antibacterial tetracycline compounds to inhibit cancer growth. The method is particularly effective to inhibit the establishment growth and metastasis of solid tumors such as tumor...

A method for inhibiting the activity of serine proteinase in biological systems includes administering to the system a serine proteinase inhibitory amount of a hydrophobic 4-de(dimethylamino) tetracycline. The method reduces tissue destruction during inflammation resulting from the activity of human leukocyte elastase. The method is used for pharmaceutical and cosmetic purposes.

A method for improving the healing response of epithelialized tissue (e.g. skin mucosae) to acute traumatic injury is disclosed. The method includes employing a tetracycline compound having substantially no antibacterial activity to improve the capacity of the epithelialized tissue to heal acute wounds. Specifically the method involve s increasing the rate of collagen accumulation of the healing epithelialized tissue above that associated with wound healing in the individual. The method decrease...

The invention relates to methods for protecting and/or treating a mammal at risk of acquiring a condition associated with bacteria that produce a calmodulin exotoxin a metalloproteinase exotoxin or both by administering a non-antibacterial tetracycline formulation.